three-dimensional structures of both the wild-type and mutant pigt Search Results


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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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<t>Meningococcal</t> <t>biofilm</t> formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).
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Image Search Results


Meningococcal biofilm formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).

Journal: Infection and Immunity

Article Title: Identification of Genes Involved in Neisseria meningitidis Colonization

doi: 10.1128/IAI.00421-13

Figure Lengend Snippet: Meningococcal biofilm formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening. During the screening process, wild-type and mutant strains were grown over T84 epithelial cells for 18 h. At least three independent experiments were performed. The results are normalized as a percentage of the mean biomass of the wild-type strain, which was set to 100%. Error bars indicate the standard errors of the mean (SEM). ***, P < 0.005; **, P < 0.01; *, P < 0.05 (Student t test).

Article Snippet: Complementation reversed the colonization defect observed in both mutant strains ( and ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig 2 caption a7 Meningococcal biofilm formation over epithelial cells. (A) Three-dimensional biofilm structures reconstructed with Imaris software of wild-type and five mutant strains grown over T84 epithelial cells for 18 h. Representative images of experiments performed on at least three different occasions are shown. (B) Analyses of biomass and average thickness using COMSTAT software of wild-type and five mutant strains identified in STM screening.

Techniques: Software, Mutagenesis